inverted spinning disk confocal microscope (Nikon)

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Nikon inverted spinning disk confocal microscope
Inverted Spinning Disk Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 40098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/inverted+spinning+disk+confocal+microscope/NIS-Elements/pm41844625-288-9-14
Average 99 stars, based on 40098 article reviews

inverted spinning disk confocal microscope - by Bioz Stars, 2026-10

99/100 stars

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Confocal Microscopy:

Article Title: Macrophages migrate persistently and directionally upon entering 2D confinement in the presence of extracellular matrix
Article Snippet: .. Confocal microscopy: a Nikon inverted spinning disk confocal microscope outfitted with an EMCCD camera (Yokogawa) and 60× Plan Apo 1.4 NA oil objective was used to image the agarose-dextran confinement of Lifeact-Scarlet expressing macrophages. ..

Microscopy:

Article Title: TMED9 coordinates the clearance of misfolded GPI-anchored proteins out of the ER and into the Golgi.
Article Snippet: Cells were blocked with 5% normal goat serum in PBS (Thermofisher, 31872) and stained with mouse monoclonal αTMED9 (Novus, NBP3–33028) primary antibody at 1:500 followed by Goat anti-Mouse IgG, Alexa Fluor 660 (ThermoFisher Scientific, A-21055) secondary antibody diluted at 1:2,000. .. Images were acquired using a Nikon inverted spinning disk confocal microscope equipped with a Yokogawa CSU-X1 Spinning Disk, EMCCD camera, 60× Plan Apo 1.40 NA oil/ 0.13-mm WD, Tokai live-cell incubator, Perfect Focus system to prevent focus drift, lasers (405, 445, 488, 514, 561, and 647 nm), emission filter sets (455/50, 480/40, 525/36, 540/30, 605/70, and 700/75), and dichroic mirror sets (405/488/561/640 and 440/514/561). ..

Article Title: Macrophages migrate persistently and directionally upon entering 2D confinement in the presence of extracellular matrix.
Article Snippet: .. Confocal microscopy: A Nikon inverted spinning disk confocal microscope outfitted with an EMCCD camera (Yokogawa) and 60x Plan Apo 1.4 NA oil objective was used to image the agarose-dextran confinement of Lifeact-Scarlet expressing macrophages. ..

Article Title: Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair.
Article Snippet: .. Imaging and analysis of immunostained tissue sections: All images were acquired using an inverted spinning disk confocal microscope (Nikon, NISElements Viewer 5.2 software), with a 60x magnification objective (in air) (NA=0.95; WD=200 μm). .. All the images were acquired with the following parameters: Z-step: 1 μm; DAPI laser power: 30%, exposure 500 ms; 488 laser power: 50%, exposure 700 ms; 555 laser power: 25%, exposure 500 ms. All imaging data were analysed using ImageJ software.

Article Title: Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair
Article Snippet: The antibodies used for immunofluorescence were: H3 (mouse, 1:250; Cell Signaling, 14269S), H3K9ac (Rabbit, 1:250; Abcam, ab10812), H3K14ac (Rabbit, 1:250; Abcam, ab52946), H3K18ac (Rabbit, 1:250; Invitrogen, PA-585523), H3K23ac (Rabbit, 1:250; Sigma, SAB5600023), H3K27ac (Rabbit, 1:250; Abcam, ab4729), H3K36ac (Rabbit, 1:250; ThermoFisher, MA5-24672), H4 (mouse, 1:250; ThermoFisher, MA550233), H4K5ac (Rabbit, 1:250; Invitrogen, SAB5600018), H4K8ac (Rabbit, 1:250; Sigma, SAB5600019), H4K12ac (Rabbit, 1:250; Abcam, ab46983), H4K16ac (Rabbit, 1:250; Invitrogen, 720083), RNAPolS2P (Rat, 1:250; Abcam, ab252855), Osteopontin (Mouse, 1:50; Santa Cruz, sc21742), Cadherin-13 (Rabbit, 1:50; Abcam, ab36905), KAT7 (Rabbit, 1:250; Abcam, ab70183), Cleaved Caspase 3 (Rabbit 1/250; Cell Signaling, 9579). .. Imaging after 3D immunostaining was performed using an inverted spinning disk confocal microscope (Nikon, NIS-Elements Viewer 5.2 software). ..

Article Title: Supporting Information Social memory in female mice is rapidly modulated by 17-estradiol through ERK and Akt modulation of synapse formation
Article Snippet: Finally, sections air-dried at RT for 1 h before coverslipping with mounting medium containing DAPI (Prolong Gold; Thermofisher). .. Confocal image acquisition and analysis Confocal images of the CA1 region of the hippocampus for quantification of synaptic puncta were acquired at the Wohl Cellular Imaging Centre using an Inverted Spinning Disk confocal microscope (Nikon, Japan) and 60x oil immersion lens objective (NA 1.4). ..

Article Title: Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair
Article Snippet: .. All images were acquired using an inverted spinning disk confocal microscope (Nikon, NIS-Elements Viewer 5.2 software), with a 60x magnification objective (in air) (NA=0.95; WD=200 μm). .. All the images were acquired with the following parameters: Z-step: 1 μm; DAPI laser power: 30%, exposure 500 ms; 488 laser power: 50%, exposure 700 ms; 555 laser power: 25%, exposure 500 ms. All imaging data were analysed using ImageJ software.

Expressing:

other:

Article Title: Wnt-presenting materials sustain H3K14-acetylation in human skeletal stem cells for tissue engineering and bone repair.
Article Snippet: Cells were fixed in 4% paraformaldehyde (PFA) for 20 min at room temperature and subsequently processed for apoptosis detection using the Click-iT Plus TUNEL Assay Kit (ThermoFisher, C10619).

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inverted spinning disk confocal microscope (Nikon)

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